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naei restriction enzymes  (New England Biolabs)


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    Structured Review

    New England Biolabs naei restriction enzymes
    Naei Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/naei+restriction+enzymes/NaeI/pmc12752508-171-13-16
    Average 94 stars, based on 130 article reviews
    naei restriction enzymes - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Xrn1p Acts at Multiple Steps in the Budding-Yeast RNAi Pathway to Enhance the Efficiency of Silencing
    Article Snippet: .. To construct pRS403-ScerURA3hp-HIS3-PEST, the integrating plasmid containing the URA3 -silencing construct, the N. castellii genomic sequence downstream of the ORF NCAS0C02950 was amplified (primers: Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 ( ) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). .. In addition, the PEST sequence from S. cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described , which increased the stringency of the selection.

    Plasmid Preparation:

    Article Title: Xrn1p Acts at Multiple Steps in the Budding-Yeast RNAi Pathway to Enhance the Efficiency of Silencing
    Article Snippet: .. To construct pRS403-ScerURA3hp-HIS3-PEST, the integrating plasmid containing the URA3 -silencing construct, the N. castellii genomic sequence downstream of the ORF NCAS0C02950 was amplified (primers: Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 ( ) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). .. In addition, the PEST sequence from S. cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described , which increased the stringency of the selection.

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate.
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences (Lmx1>Lmx1::VP64) was obtained by first cloning Lmx1 regulatory sequences (Lemaire et al., 2021) into an expression vector containing the VP64 activation domain (Gainous et al., 2015) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences ( Lmx1 > Lmx1::VP64 ) was obtained by first cloning Lmx1 regulatory sequences ( ) into an expression vector containing the VP64 activation domain ( ) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Sequencing:

    Article Title: Xrn1p Acts at Multiple Steps in the Budding-Yeast RNAi Pathway to Enhance the Efficiency of Silencing
    Article Snippet: .. To construct pRS403-ScerURA3hp-HIS3-PEST, the integrating plasmid containing the URA3 -silencing construct, the N. castellii genomic sequence downstream of the ORF NCAS0C02950 was amplified (primers: Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 ( ) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). .. In addition, the PEST sequence from S. cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described , which increased the stringency of the selection.

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate.
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences (Lmx1>Lmx1::VP64) was obtained by first cloning Lmx1 regulatory sequences (Lemaire et al., 2021) into an expression vector containing the VP64 activation domain (Gainous et al., 2015) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences ( Lmx1 > Lmx1::VP64 ) was obtained by first cloning Lmx1 regulatory sequences ( ) into an expression vector containing the VP64 activation domain ( ) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Amplification:

    Article Title: Xrn1p Acts at Multiple Steps in the Budding-Yeast RNAi Pathway to Enhance the Efficiency of Silencing
    Article Snippet: .. To construct pRS403-ScerURA3hp-HIS3-PEST, the integrating plasmid containing the URA3 -silencing construct, the N. castellii genomic sequence downstream of the ORF NCAS0C02950 was amplified (primers: Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 ( ) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). .. In addition, the PEST sequence from S. cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described , which increased the stringency of the selection.

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate.
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences (Lmx1>Lmx1::VP64) was obtained by first cloning Lmx1 regulatory sequences (Lemaire et al., 2021) into an expression vector containing the VP64 activation domain (Gainous et al., 2015) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences ( Lmx1 > Lmx1::VP64 ) was obtained by first cloning Lmx1 regulatory sequences ( ) into an expression vector containing the VP64 activation domain ( ) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Clone Assay:

    Article Title: Xrn1p Acts at Multiple Steps in the Budding-Yeast RNAi Pathway to Enhance the Efficiency of Silencing
    Article Snippet: .. To construct pRS403-ScerURA3hp-HIS3-PEST, the integrating plasmid containing the URA3 -silencing construct, the N. castellii genomic sequence downstream of the ORF NCAS0C02950 was amplified (primers: Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 ( ) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). .. In addition, the PEST sequence from S. cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described , which increased the stringency of the selection.

    Polymerase Chain Reaction:

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate.
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences (Lmx1>Lmx1::VP64) was obtained by first cloning Lmx1 regulatory sequences (Lemaire et al., 2021) into an expression vector containing the VP64 activation domain (Gainous et al., 2015) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..

    Article Title: Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate
    Article Snippet: The transgene expressing the constitutively active Lmx1 transcription factor controlled by Lmx1 regulatory sequences ( Lmx1 > Lmx1::VP64 ) was obtained by first cloning Lmx1 regulatory sequences ( ) into an expression vector containing the VP64 activation domain ( ) using the NotI and AscI restriction enzymes (New England Biolabs). .. The coding sequence of Lmx1 was then PCR amplified, digested with NotI and NaeI restriction enzymes (New England Biolabs), and ligated (T4 DNA ligase, Promega) into the previously obtained plasmid digested with the same enzymes. ..



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